Bacterial Genetics
Conjugation:
 
 
 
 
 
 
 
Auxotrophy:
 
 
 
 
 
 
Problems:
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
Transduction:
 
 
 
 
 
 
Problems
 
 
 
 
 
 
 
 
Tutorial
Genetic exchange in bacteria
F+ versus F- cells
Animation of F+ x F-
Integration of F+ (animation)
Hfr strains
Animation of Hfr x F-
 
Tutorial
Biochemical pathways
Gene mutation
Replica plating video
Antibiotic markers
Sugar markers
 
1. Selectable markers
Amino acid markers
Antibiotic markers
Sugar markers
Replica plates
Genotypes
 
2. F+, F- and Hfr cells
Many, few or no recombinants
Predicting cell type from data
 
3. Interrupted mating
Selection for exconjugants
Time of entry
Map in minutes from origin of transfer
 
4. Natural Gradient of Transfer
Auxotrophic marker to select against Hfr parent
Selection of two markers per plate
Gene order using data
 
5. Multiple Hfrs
Circular map
 
Tutorial
Phage Life Cycle (animation)
Transducing phage
Donor
Recipient
Co-transduction
 
1. Two Factor Crosses
Co-transduction with five auxotrophic markers
Co-transduction frequencies
Gene order
2. Three Factor Crosses (markers in cis)
Genotype frequencies from replica plates
Co-transduction frequencies
3. Simulation of Three Factor Cross (markers in trans)
Choice of donor or recipient strain
Choice of selectable media
Replica plate data
Final map determination
4. Reciprocal Crosses
Two crossovers vs four crossovers
Gene order with respect to nearby marker