Molecular Biology
Gene expression:
 
 
 
 
Gene Cloning:
 
 
 
 
 
 
 
 
 
 
 
 
 
 
Problems:
 
 
 
 
 
 
 
 
 
Tutorials
Transcription
Splicing
Translation
 
Tutorials
DNA Sequencing
Nucleotide addition
Inhibition by dideoxynucleotides
Animation of DNA sequencing
 
Southern blot
Restriction digest of total DNA
Transfer of fragments to nitrocellulose (video)
Radioactive probe
Autoradiogram
 
PCR
PCR animation
 
1. Cloning Hemoglobin gene from patient
Splicing defect indicated
Wild type gene available
Choice of insert, vector, method of identification
 
2. Cloning DNA polymerase gene
Given pure protein
Choice of insert, vector, method of identification
 
3. Cloning Polydactyly gene
Given linked RFLP
Choice of insert, vector, method of identification